Save
Standardization of Light Transmission Aggregometry for the diagnosis of inherited platelet function disorders: First inter-laboratory external quality assessment in Germany and Austria
ISTH Academy. Althaus K. Jul 9, 2019; 264513; PB1322
Dr. Karina Althaus
Dr. Karina Althaus
Access to Reserved content is available to attendees of the Congress until the end of the year and always available for full ISTH members.

Click here to join ISTH or renew your membership.

Abstract
Discussion Forum (0)
Rate & Comment (0)

PB1322

Standardization of Light Transmission Aggregometry for the Diagnosis of Inherited Platelet Function Disorders: First Inter-laboratory External Quality Assessment in Germany and Austria

K. Althaus1, B. Zieger2, T. Bakchoul3,4, K. Jurk5, Thromkid-Plus Studiengruppe (GTH)
1Universitätsklinikum Tübingen, Center for Clinical Transfusion Medicine, Tuebingen, Germany, 2University of Freiburg, Department of Pediatrics and Adolescent Medicine, Division of Pediatric Hematology and Oncology, Medical Center, Freiburg, Germany, 3University Hospital Tuebingen, Center for Clinical Transfusion Medicine, Tuebingen, Germany, 4Medical Faculty of Tuebingen, Transfusion Medicine, Tuebingen, Germany, 5University Medical Center of the Johannes Gutenberg University, Center for Thrombosis and Hemostasis (CTH), Mainz, Germany

Main Topic: Diagnostics and OMICs
Category: Laboratory Diagnostics – Platelet

Background: Several in vitro platelet function tests are available for the diagnosis of inherited platelet function disorders. Currently, the light transmission aggregometry (LTA) is recommended as one of first-step tests, which are available in most clinical centers. Although the LTA is accepted as a 'gold standard' assay for the evaluation of platelet function, its standardization in the clinical practice is still challenging. The GTH-based THROMKID-Plus Study Group has created a standard and a quality management of the LTA and performed an inter-laboratory survey in Germany and Austria.
Aims: The aim was to provide a practical, achievable and affordable external quality control, with easy handling and high reliability of standardization.
Methods: Five different agonists (stable at 4°C) were selected according to the SSC/ISTH recommendations and shipped for three different sets (healthy control, simulated platelet function disorders) to 15 specialised laboratories in Germany and Austria. Agonists were analysed in the APACT or PAP4/8 aggregometer using platelet-rich plasma of a healthy donor from each lab. In addition, lab-internal platelet agonists were tested in platelet-rich plasma from a healthy donor.
Results: All laboratories (9 APACT/6 PAP4/PAP8) showed very consistent data regarding the maximum percentage of aggregation induced by the tested agonists and identified the differential diagnosis of the simulated platelet function disorders with one exception, which was due to technical problems. In contrast, there was a high variability of the lab-internal inductors regarding reagent type, concentrations and pathological cut-off values.
Conclusions: The shipment of agonists is suitable for an inter-laboratory survey of LTA. However, there are still persistent needs for standardisation of agonist reagents and their concentration ranges as well as for definition of reference ranges.

Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.



Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.


Save Settings