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Assessment of whole blood coagulation with a microfluidic dielectric sensor
Author(s): ,
D. Maji
Affiliations:
Electrical Engineering and Computer Science Department, Case Western Reserve University, Cleveland, USA
,
M. De La Fuente
Affiliations:
Department of Pharmacology, Case Western Reserve University, Cleveland, USA
,
E. Kucukal
Affiliations:
Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, USA
,
U. D. S. Sekhon
Affiliations:
Biomedical Engineering Department, Case Western Reserve University, Cleveland, USA
,
A. H. Schmaier
Affiliations:
Department of Medicine, Hematology and Oncology Division, University Hospitals Cleveland Medical Center, Cleveland, USA. Department of Medicine, Hematology and Oncology Division, Case Western Reserve University School of Medicine, Cleveland, USA
,
A. Sen Gupta
Affiliations:
Biomedical Engineering Department, Case Western Reserve University, Cleveland, USA
,
U. A. Gurkan
Affiliations:
Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, USA. Biomedical Engineering Department, Case Western Reserve University, Cleveland, USA
,
M. T. Nieman
Affiliations:
Department of Pharmacology, Case Western Reserve University, Cleveland, USA
,
E. X. Stavrou
Affiliations:
Department of Medicine, Louis Stokes Veterans Administration Medical Center, Cleveland, USA. Department of Medicine, Hematology and Oncology Division, Case Western Reserve University School of Medicine, Cleveland, USA
,
P. Mohseni
Affiliations:
Electrical Engineering and Computer Science Department, Case Western Reserve University, Cleveland, USA
Correspondence: Pedram Mohseni, Electrical Engineering and Computer Science Department, Case Western Reserve University, 2123 Martin Luther King Jr. Drive, Cleveland, OH, USA|Tel.: +1 216 368 5263|E‐mail: pedram.mohseni@case.edu
M. A. Suster
Affiliations:
Electrical Engineering and Computer Science Department, Case Western Reserve University, Cleveland, USA
ISTH Academy. Mohseni P. Oct 4, 2018; 234178
Pedram Mohseni
Pedram  Mohseni

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Background
Rapid point‐of‐care (POC) assessment of hemostasis is clinically important in patients with a variety of coagulation factor and platelet defects who have bleeding disorders.
Objective
To evaluate a novel dielectric microsensor, termed ClotChip, which is based on the electrical technique of dielectric spectroscopy for rapid, comprehensive assessment of whole blood coagulation.
Methods
The ClotChip is a three‐dimensional, parallel‐plate, capacitive sensor integrated into a single‐use microfluidic channel with miniscule sample volume (< 10 μL). The ClotChip readout is defined as the temporal variation in the real part of dielectric permittivity of whole blood at 1 MHz.
Results
The ClotChip readout exhibits two distinct parameters, namely, the time to reach a permittivity peak (T) and the maximum change in permittivity after the peak (Δε), which are, respectively, sensitive towards detecting non‐cellular (i.e. coagulation factor) and cellular (i.e. platelet) abnormalities in the hemostatic process. We evaluated the performance of ClotChip using clinical blood samples from 15 healthy volunteers and 12 patients suffering from coagulation defects. The ClotChip T parameter exhibited superior sensitivity at distinguishing coagulation disorders as compared with conventional screening coagulation tests. Moreover, the ClotChip Δε parameter detected platelet function inhibition induced by aspirin and exhibited strong positive correlation with light transmission aggregometry.
Conclusions
This study demonstrates that ClotChip assesses multiple aspects of the hemostatic process in whole blood on a single disposable cartridge, highlighting its potential as a POC platform for rapid, comprehensive hemostatic analysis.
Keyword(s)
blood coagulation, blood coagulation disorders, blood coagulation factor inhibitors, blood coagulation tests, platelet function tests
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