Save
Elevated plasma levels of P‐selectin glycoprotein ligand‐1‐positive microvesicles in patients with unprovoked venous thromboembolism
Author(s): ,
S. Jamaly
Affiliations:
K.G. Jebsen Thrombosis Research and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, Tromsø, Norway
,
M. G. Basavaraj
Affiliations:
K.G. Jebsen Thrombosis Research and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, Tromsø, Norway
,
I. Starikova
Affiliations:
K.G. Jebsen Thrombosis Research and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, Tromsø, Norway. Division of Internal Medicine, University Hospital of North Norway, Tromsø, Norway
,
R. Olsen
Affiliations:
Advanced Microscopy Core Facility, Institute of Medical Biology, Faculty of Health Sciences, UiT – The Arctic University of Norway, Tromsø, Norway
,
S. K. Brækkan
Affiliations:
K.G. Jebsen Thrombosis Research and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, Tromsø, Norway. Division of Internal Medicine, University Hospital of North Norway, Tromsø, Norway
J.‐B. Hansen
Affiliations:
K.G. Jebsen Thrombosis Research and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, Tromsø, Norway. Division of Internal Medicine, University Hospital of North Norway, Tromsø, Norway
Correspondence: John‐Bjarne Hansen, K.G. Jebsen Thrombosis and Expertise Center (TREC), Department of Clinical Medicine, UiT – The Arctic University of Norway, N‐9037 Tromsø, Norway|Tel.: +47 7762 5105|E‐mail: john-bjarne.hansen@uit.no
ISTH Academy. Hansen J. Aug 2, 2018; 227424
John-Bjarne Hansen
John-Bjarne Hansen

Access to Premium content is currently a membership benefit.


Click here to join ISTH or renew your membership.

Journal Abstract
Discussion Forum (0)
Rate & Comment (0)


Background
Microvesicles (MVs) express antigens from their parental cells and have a highly procoagulant surface. Animal studies suggest that P‐selectin glycoprotein ligand‐1‐positive (PSGL‐1) MVs play a role in the pathogenesis of venous thromboembolism (VTE).
Objective
The aim of this study was to determine plasma levels, the cellular origin and the morphological characteristics of PSGL‐1 MVs in patients with unprovoked VTE.
Methods
We conducted a population‐based case–control study in 20 patients with a history of unprovoked VTE and 20 age‐ and sex‐matched healthy controls recruited from the general population. Plasma levels, the cellular origin and the morphological characteristics of PSGL‐1 MVs were evaluated using flow cytometry, electron microscopy and confocal microscopy.
Results
Plasma levels of PSGL‐1 MVs were associated with increased risk of VTE. The odds ratio per one standard deviation increase in PSGL‐1 MVs was 3.11 (95% confidence interval [CI], 1.41–6.88) after adjustment for age and sex, and 2.88 (95% CI, 1.29–6.41) after further adjustment for body mass index. The PSGL‐1 MVs originated mainly from monocytes and endothelial cells determined by double staining with markers of parental cells using flow cytometry and transmission electron microscopy. Scanning electron microscopy of PSGL‐1‐labeled plasma‐derived MVs displayed dominantly spherical vesicles that varied between 50 and 300 nm in diameter.
Conclusions
Increased plasma levels of PSGL‐1 MVs are associated with the risk of unprovoked VTE. Large population‐based prospective studies are required to validate our findings.
Keyword(s)
case–control studies, cell‐derived microparticles, platelets, P‐selectin, venous thromboembolism
Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.



Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.


Save Settings