Measurement of tissue factor activity in extracellular vesicles from human plasma samples
Author(s): ,
Yohei Hisada
Affiliations:
Division of Hematology and Oncology, Thrombosis and Hemostasis Program, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill
Nigel Mackman
Affiliations:
Division of Hematology and Oncology, Thrombosis and Hemostasis Program, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill. Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill
Correspondence |Nigel Mackman, Division of Hematology and Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC.|Email: nmackman@med.unc.edu
ISTH Academy. Mackman N. Jan 15, 2019; 273420
Nigel Mackman
Nigel Mackman
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Abstract
Tissue factor (TF) is the cellular receptor for plasma factor (F) VII/FVIIa and triggers blood coagulation. Extracellular vesicles (EVs) are small membrane vesicles that are released from activated cells and tumor cells. Different cell types, including activated monocytes and tumors cells release TF‐positive EVs into the circulation. We developed an assay to measure levels of TF activity in EVs isolated from human plasma samples. We and others have used this assay to demonstrate increased levels of EV TF activity in a variety of diseases associated with thrombosis, including cancer. These studies suggest that EV TF can be used as a biomarker of thrombotic risk. The strength of this laboratory assay is that it is relatively sensitive and specific. However, the limitations of the assay are that it is labor intensive and the coefficient of variation is too high for it to be used as a clinical assay.
Keyword(s)
biomarker, extracellular vesicles, plasma, thrombosis, tissue factor
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