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Natural killer cells induce neutrophil extracellular trap formation in venous thrombosis
Author(s): ,
F.‐R. Bertin
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada. School of Veterinary Science, The University of Queensland, Gatton, Australia
,
R. N. Rys
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada
,
C. Mathieu
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada
,
S. Laurance
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada
,
C. A. Lemarié
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada. Department of Medicine, Sir Mortimer B. Davis‐Jewish General Hospital, McGill University, Montreal, Canada. EA3878 (GETBO), University Hospital of Brest, European University of Occidental Brittany, Brest, France
Correspondence: Catherine A. Lemarié, EA3878 – GETBO, Université de Bretagne Occidentale, Faculté de Médecine, 22 rue Camille Desmoulins, 29238 Brest, France|Tel.: +33 29 801 6956|E‐mail: catherine.lemarie@univ-brest.fr
M. D. Blostein
Affiliations:
Lady Davis Institute for Medical Research, Montreal, Canada. Department of Medicine, Sir Mortimer B. Davis‐Jewish General Hospital, McGill University, Montreal, Canada
ISTH Academy. A. Lemarié C.
Feb 8, 2019; 273384
Catherine A. Lemarié
Catherine  A. Lemarié
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Background
Neutrophils contribute to venous thrombosis through the release of neutrophil extracellular traps (NETs), but the mechanism triggering their formation remains unclear. In vitro data show that interferon (IFN)‐γ induces the formation of NETs.
Objectives
To determine whether IFN‐γ and the transcription factor T‐box expressed on T cells (Tbet) promote venous thrombosis through neutrophil activation.
Methods
Venous thrombosis was induced by flow restriction in the inferior vena cava in IFN‐γ, Tbet or wild‐type (WT) mice. After 48 h, thrombus size was measured by the use of high‐frequency ultrasound. NET formation was determined by immunofluorescence.
Results and Conclusions
Thrombus formation was reduced in Tbet and IFN‐γ mice, suggesting that Tbet/IFN‐γ‐expressing cells are required for venous thrombosis. The number of NETs formed during thrombosis was significantly lower in Tbet and IFN‐γ mice. NET formation was also decreased in WT mice treated with an IFN‐γ‐blocking antibody. Injection of recombinant IFN‐γ into IFN‐γ mice rescued the phenotype. Natural killer (NK) cells were specifically depleted prior to venous thrombosis induction. NK cell depletion results in decreased NET formation and smaller thrombi, suggesting that NK cells are required for thrombus development. In depleted mice, adoptive transfer of WT NK cells induced a similar thrombosis burden as in WT mice. In contrast, adoptive transfer of IFN‐γ NK cells resulted in thrombi similar in size to those in depleted mice. In vitro, we showed that WT neutrophils released fewer NETs when they were cocultured with IFN‐γ NK cells. This study demonstrates that NK cell‐dependent IFN‐γ production is crucial for thrombus development by promoting the formation of NETs by neutrophils.
Keyword(s)
extracellular traps, IFN‐γ, natural killer cells, neutrophils, venous thrombosis
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