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An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: the COMPASS‐VWF study
Author(s): ,
A. Szederjesi
Affiliations:
Szent István and Szent László Hospital, Budapest, Hungary
,
L. Baronciani
Affiliations:
Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, A. Bianchi Bonomi Hemophilia and Thrombosis Center, Milan, Italy
,
U. Budde
Affiliations:
Laboratory of Hemostasis, University Hospital Hamburg, Hamburg, Germany
,
G. Castaman
Affiliations:
Center for Bleeding Disorders, Careggi University Hospital, Florence, Italy
,
A. S. Lawrie
Affiliations:
Haemostasis Research Unit, University College London, London, UK
,
Y. Liu
Affiliations:
Winship Cancer Institute, Emory University, Atlanta, USA
,
R. Montgomery
Affiliations:
MFRC, Medical College of Wisconsin, Milwaukee, USA
,
F. Peyvandi
Affiliations:
Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, A. Bianchi Bonomi Hemophilia and Thrombosis Center, Milan, Italy
,
R. Schneppenheim
Affiliations:
University Medical Center, Hamburg‐Eppendorf, Hamburg, Germany
,
A. Várkonyi
Affiliations:
Szent István and Szent László Hospital, Budapest, Hungary
,
J. Patzke
Affiliations:
Department of Assay Development, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany
I. Bodó
Affiliations:
Szent István and Szent László Hospital, Budapest, Hungary. Winship Cancer Institute, Emory University, Atlanta, USA
Correspondence: Imre Bodó, 3rd Department of Internal Medicine, Semmelweis University, 4 Kútvölgyiút, Budapest 1125, Hungary|Tel.: +36 30 450 4483|E‐mail: bodoimre.md@gmail.com
ISTH Academy. Bodó I. Aug 2, 2018; 227440
Dr. Imre Bodó
Dr. Imre Bodó

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Background
Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other.
Methods
The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well‐characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study.
Results
All tests correlated well with VWF:RCo activity (r‐values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL. The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3–4 and 3–6 IU dL range, respectively. Inter‐laboratory variation was also improved for most new assays.
Conclusion
All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS‐VWF study will assist the VWF community in interpreting and comparing activity results.
Keyword(s)
clinical laboratory techniques, hemorrhagic disorders, hemostasis, von Willebrand disease, von Willebrand factor
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